Impact of albumin corona on mucoadhesion and antimicrobial activity of carvacrol loaded chitosan nano-delivery systems under simulated gastro-intestinal conditions

Emerging antibiotic resistance in pathogens has posed considerable challenges to explore and examine the natural antimicrobials (NAMs). Due labile nature of NAMs, nano-delivery systems (NDS) are required protect them from physiological degradation allow controlled delivery targeted site infection. In this study, corona modified NDS were developed using bovine serum albumin (BSA) on a chitosan core (CS) for sustained carvacrol (CAR), antimicrobial agent, intestine. The optimal nano-formulations (CS-NDS) (BSA-CS-NDS) fabricated with an average diameter 52.4 ± 10.4 nm 202.6 6 nm, respectively. A shift zeta-potential (ZP) positive (+21 3.6 mV) negative values (−18 2.6 confirmed electrostatic deposition BSA CS core. Under influence various simulated gastrointestinal conditions, provided extra stability (ZP -38.5 mV), by ensuring delayed release limited gastric conditions. Mucoadhesive studies quartz crystal microbalance dissipation (QCM-D) revealed that reduced mucoadhesion at pH, which enabled effective CAR intestinal phase successful eradication Salmonella enterica . • having ionic gelation ultrasonication. Corona increased particle during vitro digestion. Core-corona nano-antimicrobials successfully inhibited enteric pathogen i e